041 Increased Interleukin-36γ in keratinocytes may induce epithelial-mesenchymal transition in patients with systemic sclerosis

Systemic sclerosis (SSc) is a connective tissue disease characterized by autoimmunity, vasculopathy, and fibrosis. Several studies demonstrated the involvement of various cytokines including IL-1 in SSc. IL-36 cytokines, subgroup family, comprise IL-36α, β, γ, IL-36Ra, IL-38. γ are known to promote inflammatory response, which mainly produced epithelial cells. Whereas, IL-36Ra as well IL-38 may have anti-inflammatory effect. Recent reports suggest that epidermis play an important role pathogenesis SSc, although family still unclear. Thus, we focused on regarding its level function expressions were evaluated immunohistochemistry skin from SSc patients healthy controls, for IL-36γ western blot analysis. Serum levels ELISA 61 with controls. As results, expression significantly higher compared controls sera also had increased IL-36γ. To analyze cultured keratinocytes stimulated IL-36γ, epithelial-mesenchymal transition (EMT)-related factors analyzed qRT-PCR Immunofluorescence. Expression E-cadherin downregulated while Slug Vimentin IL-36γ-stimulated or without TGFβ than Furthermore, fibroblasts presence induced Egr-1 expression. In conclusion, be involved fibrotic inducing EMT.